CJC-1295 without DAC (Mod GRF 1-29)

CJC-1295 names two molecules. The form on this page carries no drug affinity complex and is also written Mod GRF 1-29, with a calculated mass of 3367.90 g/mol. The DAC form is a different molecule of about 3647 g/mol. Neither holds a marketing authorisation in the UK or the EU, and a listing without a mass has not said which is being sold.

Nomenclature: CJC-1295, Mod GRF 1-29, DAC and no-DAC

The parent sequence is GHRH(1-29), the first 29 residues of growth hormone-releasing hormone. The modified analogue substitutes four positions - D-Ala at 2, Gln at 8, Ala at 15 and Leu at 27 - and it circulates in this trade under at least four names: CJC-1295 without DAC, CJC-1295 no-DAC, Mod GRF 1-29 and Modified GRF (1-29). All four mean the molecule described on this page, mass 3367.90 g/mol, C₁₅₂H₂₅₂N₄₄O₄₂, with a C-terminal amide.

The name CJC-1295 on its own more often refers to the DAC form, in which the peptide carries a maleimidopropionic linker that binds serum albumin. That is a larger molecule: the public record lists it at about 3647 g/mol, PubChem CID 91971820, CAS 446262-90-4. Two molecules, one abbreviation, roughly 280 daltons apart. The first question to put to any listing is therefore which of the two it is describing, and the answer that settles it is a mass rather than a name.

The parent compound has been described in the endocrinology literature since the mid-2000s - one early report appeared in the Journal of Clinical Endocrinology and Metabolism in 2006 (PubMed 16352683). Peptorium does not summarise that work, does not transfer it to any person or animal, and runs no clinical research of its own. Naming the class the molecule belongs to is nomenclature; it is not a statement about what is in a vial.

Identity: sequence, formula, mass

The sequence is Tyr-D-Ala-Asp-Ala-Ile-Phe-Thr-Gln-Ser-Tyr-Arg-Lys-Val-Leu-Ala-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Leu-Ser-Arg-NH₂. Two details in it are worth a buyer's attention. The D-alanine at position 2 is a non-natural residue, and its presence or absence is not visible in a mass spectrum alone, because a D-amino acid weighs exactly what its L-form weighs. The C-terminus is an amide rather than a free acid, which is a difference of one dalton against the acid form and is the sort of thing a careless synthesis gets wrong.

Neither point can be settled by a purity percentage. Both are settled by a certificate that names the method, reports the measured mass beside the theoretical 3367.90 g/mol and shows the chromatogram.

Regulatory status in the UK and the European Union

Neither form holds a marketing authorisation. Nothing under either name appears in the EMA medicines database. In the UK, regulation 46 of the Human Medicines Regulations 2012 prohibits selling, supplying or offering an unauthorised medicinal product, and regulation 2 defines a medicinal product partly by presentation: what the seller says about a substance can bring it inside the definition without the substance changing. Sporting bodies maintain their own lists on their own schedule, and those are read at the federation rather than at a shop.

"Research use only" is a purpose, not a permission

It states that the seller offers the material for laboratory work rather than for use in people. It is not a legal category in the UK or the EU, not a licence, and not evidence that any authority has looked at anything. It says nothing about how the material was made or what was measured, and a page that uses it in place of those facts has answered a different question from the one asked.

Purity and peptide content

Purity by HPLC is a ratio: the area of the main peak as a share of all peak area detected. Peptide content is a quantity: the share of the vial's mass that is peptide at all, the rest being residual water and the counter-ions from synthesis, typically acetate or trifluoroacetate. Water is measured separately by Karl Fischer titration. A 29-residue chain is long enough that deletion sequences - chains missing a residue - are the impurities to expect, and they sit close to the target in mass, so a headline percentage with no impurity table behind it is a weak document. The purity and peptide content guide and the certificate guide set out what a full report contains.

What Peptorium publishes about this material

This page publishes identity: the sequence, the molecular formula and a calculated mass of 3367.90 g/mol for the no-DAC form. Each of those can be checked against a public register rather than taken on trust. Purity, peptide content and water describe one batch rather than a compound. Those figures belong on a certificate of analysis issued by the laboratory that tested that batch, and they travel with the material itself.

What to ask before buying

• DAC or no-DAC? Ask for the theoretical mass, not the abbreviation.
• Is the C-terminus an amide, and does the certificate's measured mass agree with 3367.90 g/mol for the no-DAC form?
• Is there a batch number, and a certificate for that batch alone?
• Are impurity peaks listed, with a chromatogram, rather than one headline figure?
• Is peptide content reported separately, with water by Karl Fischer?
• Are the method and date named, and is the report traceable to the laboratory that produced it?

What is not known

• Whether a vial contains the D-Ala² analogue or an unmodified sequence cannot be settled by mass alone, and most certificates do not address it.
• What a particular vial holds cannot be known without analysing it.
• Storage guidance for a lyophilisate is convention rather than the result of a stability study on a given lot.

Frequently asked questions

The lyophilisate is dissolved in bacteriostatic water, and the volume of solvent fixes the concentration of the solution; the peptide calculator converts between the two and recommends no quantity for any purpose. Material described here is for laboratory and in vitro research use only - see the research declaration.